目的 研究不同采收期牡荆不同部位中牡荆素的积累动态规律和分布特性。方法 采用超声波提取法提取牡荆素,高效液相色谱法测定牡荆不同部位中牡荆素的含量,色谱柱:菲罗门柱(4.6 mm×150 mm,5 μm),流动相为甲醇-水(4∶6),流速1.0 mL·min^-1,检测波长为340 nm,柱温 35 ℃,进样量5 μL。结果 牡荆茎、花中牡荆素含量极少,牡荆叶中牡荆素的含量在不同生长时期有明显的变化规律。6~7月含量最高(1.380%~1.465%),随着牡荆花的盛开和牡荆子的形成到成熟,牡荆叶中牡荆素的含量逐渐降低,9~10月含量最低(0.720%~0.751%)。随着牡荆籽的成熟,牡荆籽中含量逐渐增加(0.435%~1.231%)。结论 牡荆不同部位牡荆素的含量随采收期不同而变化,为确定牡荆适宜采收期和药用部位提供了实验依据。

OBJECTIVE To study the accumulation dynamics and distribution law of vitexin during the whole year period in different parts of Vitex negundo var.cannabifolia (Sieb. et Zucc. ). METHODS Vitexin was obtained by ultrasonic extraction, and its content was determined by HPLC. Filodoor column (4.6 mm×150 mm,5 μm) was used. Methanol-water (volume ratio of 4;6) was used as the mobile phase at a flow rate of 1 mL·min^-1. The UV detective wavelength was set at 340 nm, injection volume was 5 μL, and the column temperature was set at 35 ℃. RESULTS The content of vitexin was low in vitex negundo branches and flowers, and had obvious regularity in different growth periods of Vitex negundo leaves. The content of vitexin was the highest (1.380%-1.465%) in June and July. Along with the blooming and formation of negundo chastetree to their maturation, the content of vitexin in Vitex negundo leaves decreased. Its content was the lowest (0.720%-0.751%) in September and October. The content of vitexin in Vitex negundo seeds increased(0.435%-1.231%)with seed maturation. CONCLUSION The content of vitexin in different parts of Vitex negundo presents a regular change in different periods. The detection of vitexin in different growth periods provides experimental basis for the selection of best collection phase and medical parts.